trypsin treatment (Thermo Fisher)

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Thermo Fisher trypsin treatment
Trypsin Treatment, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/treatment+with+trypsin/Trypsin/pm42017948-829-10-19
Average 99 stars, based on 1 article reviews

trypsin treatment - by Bioz Stars, 2026-10

99/100 stars

Images

Centrifugation:

Article Title: Human iPSC-derived alveolar macrophages reveal macrophage subtype functions of itaconate in M . tuberculosis defense
Article Snippet: .. iPSC-derived macrophages were detached by treatment with trypsin (TrypLE Express, Thermo Fisher Scientific, 12605-010) for 5 minutes before centrifugation at 400 g for 5 minutes. ..

Article Title: Human iPSC-derived alveolar macrophages reveal macrophage subtype functions of itaconate in M. tuberculosis defense.
Article Snippet: .. Flow cytometry of macrophages. iPSC-derived macrophages were detached by treatment with trypsin (TrypLE Express, Thermo Fisher Scientific, 12605-010) for 5 minutes before centrifugation at 400g for 5 minutes. ..

Article Title: Human iPSC derived alveolar macrophages reveal macrophage subtype specific functions of itaconate in M. tuberculosis host defense
Article Snippet: .. iPSC-derived macrophages were detached by treatment with trypsin (TrypLE Express (ThermoFisher Scientific; 12605-010) for 5 minutes before centrifugation at 400xg for 5 minutes. ..

Article Title: Bioorthogonal 4H-Pyrazole “Click” Reagents
Article Snippet: Preparation of HeLa Cell Lysate HeLa cells (ATCC # CCL-2) were grown in three T75 flasks to >85% confluency. .. Cells were washed with DPBS (2 × 5 mL; ThermoFisher #14190144), released from the plate by treatment with trypsin (0.25% v/v; ThermoFisher #25200056), combined, and pelleted by centrifugation at 1000 rpm for 5 min at 4 °C. ..

Flow Cytometry:

Imaging:

Article Title: Tools for Intersectional Optical and Chemical Tagging on Cell Surfaces.
Article Snippet: .. After patterning, cells were dissociated by treatment with trypsin (Thermo Fisher, 15050057), quenched with culture medium, spun down (300 g, 5 min), and resuspended in imaging buffer supplemented with 2% fetal bovine serum. .. Flow cytometry and FACS were performed at the Flow Cytometry Core at the Harvard University Bauer Core Facility, using a BD FACS Aria Fusion Cell Sorter with two rounds of 4-way sorting, and laser lines at 488, 561, and 637 nm, for TCO-AZDye488, TCO-Cy3, and TCOCy5 dye.

Article Title: Tools for intersectional optical and chemical tagging on cell surfaces
Article Snippet: Wide-area cell images were obtained at the Harvard Center for Biological Imaging, at 10 and 20x using a LSM 900 Live Cell Confocal Microscope (Zeiss). .. After patterning, cells were dissociated by treatment with trypsin (Thermo Fisher, 15050057), quenched with culture medium, spun down (300 g, 5 min), and resuspended in imaging buffer supplemented with 2% fetal bovine serum. .. Flow cytometry and FACS were performed at the Flow Cytometry Core at the Harvard University Bauer Core Facility, using a BD FACS Aria Fusion Cell Sorter with two rounds of 4-way sorting, and laser lines at 488 nm, 561 nm, and 637 nm, for TCO-AZDye488, TCO-Cy3, and TCO-Cy5 dye.

Incubation:

Article Title: Molecular degraders of extracellular proteins
Article Snippet: .. Cells were passaged by first aspirating the media, followed by treatment with trypsin (0.25%, Gibco ref 25200) and incubation at 37° C. until cells began to detach from the plate (approximately 5 minutes). ..

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